| Res. | Record | Opponent | Method | Event | Date | Round | Time | Location | Notes |
| Win | 27–8 | Dustin Poirier | Decision (unanimous) | UFC 318 | July 19, 2025 | 5 | 5:00 | New Orleans, Louisiana, United States | Return to Lightweight. Defended the symbolic UFC "BMF" title. |
| Loss | 26–8 | Ilia Topuria | KO (punches) | UFC 308 | October 26, 2024 | 3 | 1:34 | Abu Dhabi, United Arab Emirates | For the UFC Featherweight Championship. |
| Win | 26–7 | Justin Gaethje | KO (punch) | UFC 300 | April 13, 2024 | 5 | 4:59 | Las Vegas, Nevada, United States | Lightweight bout. Won the symbolic UFC "BMF" title. Performance of the Night. Fight of the Night. |
| Win | 25–7 | Jung Chan-sung | KO (punch) | UFC Fight Night: Holloway vs. The Korean Zombie | August 26, 2023 | 3 | 0:23 | Kallang, Singapore | Fight of the Night. |
| Win | 24–7 | Arnold Allen | Decision (unanimous) | UFC on ESPN: Holloway vs. Allen | April 15, 2023 | 5 | 5:00 | Kansas City, Missouri, United States | |
| Loss | 23–7 | Alexander Volkanovski | Decision (unanimous) | UFC 276 | July 2, 2022 | 5 | 5:00 | Las Vegas, Nevada, United States | For the UFC Featherweight Championship. |
| Win | 23–6 | Yair Rodríguez | Decision (unanimous) | UFC Fight Night: Holloway vs. Rodríguez | November 13, 2021 | 5 | 5:00 | Las Vegas, Nevada, United States | Fight of the Night. |
| Win | 22–6 | Calvin Kattar | Decision (unanimous) | UFC on ABC: Holloway vs. Kattar | January 16, 2021 | 5 | 5:00 | Abu Dhabi, United Arab Emirates | Fight of the Night. |
| Loss | 21–6 | Alexander Volkanovski | Decision (split) | UFC 251 | July 12, 2020 | 5 | 5:00 | Abu Dhabi, United Arab Emirates | For the UFC Featherweight Championship. |
| Loss | 21–5 | Alexander Volkanovski | Decision (unanimous) | UFC 245 | December 14, 2019 | 5 | 5:00 | Las Vegas, Nevada, United States | Lost the UFC Featherweight Championship. |
| Win | 21–4 | Frankie Edgar | Decision (unanimous) | UFC 240 | July 27, 2019 | 5 | 5:00 | Edmonton, Alberta, Canada | Defended the UFC Featherweight Championship. |
| Loss | 20–4 | Dustin Poirier | Decision (unanimous) | UFC 236 | April 13, 2019 | 5 | 5:00 | Atlanta, Georgia, United States | For the interim UFC Lightweight Championship. Fight of the Night. |
| Win | 20–3 | Brian Ortega | TKO (doctor stoppage) | UFC 231 | December 8, 2018 | 4 | 5:00 | Toronto, Ontario, Canada | Defended the UFC Featherweight Championship. Performance of the Night. Fight of the Night. |
| Win | 19–3 | José Aldo | TKO (punches) | UFC 218 | December 2, 2017 | 3 | 4:51 | Detroit, Michigan, United States | Defended the UFC Featherweight Championship. |
| Win | 18–3 | José Aldo | TKO (punches) | UFC 212 | June 3, 2017 | 3 | 4:13 | Rio de Janeiro, Brazil | Won and unified the UFC Featherweight Championship. Fight of the Night. |
| Win | 17–3 | Anthony Pettis | TKO (body kick and punches) | UFC 206 | December 10, 2016 | 3 | 4:50 | Toronto, Ontario, Canada | Won the interim UFC Featherweight Championship; Pettis missed weight (148 lb) and was ineligible to win the title. Performance of the Night. |
| Win | 16–3 | Ricardo Lamas | Decision (unanimous) | UFC 199 | June 4, 2016 | 3 | 5:00 | Inglewood, California, United States | |
| Win | 15–3 | Jeremy Stephens | Decision (unanimous) | UFC 194 | December 12, 2015 | 3 | 5:00 | Las Vegas, Nevada, United States | |
| Win | 14–3 | Charles Oliveira | TKO (neck injury) | UFC Fight Night: Holloway vs. Oliveira | August 23, 2015 | 1 | 1:39 | Saskatoon, Saskatchewan, Canada | |
| Win | 13–3 | Cub Swanson | Submission (guillotine choke) | UFC on Fox: Machida vs. Rockhold | April 18, 2015 | 3 | 3:58 | Newark, New Jersey, United States | Performance of the Night. |
| Win | 12–3 | Cole Miller | Decision (unanimous) | UFC Fight Night: Henderson vs. Thatch | February 14, 2015 | 3 | 5:00 | Broomfield, Colorado, United States | |
| Win | 11–3 | Akira Corassani | KO (punches) | UFC Fight Night: Nelson vs. Story | October 4, 2014 | 1 | 3:11 | Stockholm, Sweden | Performance of the Night. |
| Win | 10–3 | Clay Collard | TKO (punches) | UFC Fight Night: Henderson vs. dos Anjos | August 23, 2014 | 3 | 3:47 | Tulsa, Oklahoma, United States | Catchweight (149 lb) bout. |
| Win | 9–3 | Andre Fili | Submission (guillotine choke) | UFC 172 | April 26, 2014 | 3 | 3:39 | Baltimore, Maryland, United States | |
| Win | 8–3 | Will Chope | TKO (punches) | UFC Fight Night: Saffiedine vs. Lim | January 4, 2014 | 2 | 2:27 | Marina Bay, Singapore | Knockout of the Night. |
| Loss | 7–3 | Conor McGregor | Decision (unanimous) | UFC Fight Night: Shogun vs. Sonnen | August 17, 2013 | 3 | 5:00 | Boston, Massachusetts, United States | |
| Loss | 7–2 | Dennis Bermudez | Decision (split) | UFC 160 | May 25, 2013 | 3 | 5:00 | Las Vegas, Nevada, United States | |
| Win | 7–1 | Leonard Garcia | Decision (split) | UFC 155 | December 29, 2012 | 3 | 5:00 | Las Vegas, Nevada, United States | |
| Win | 6–1 | Justin Lawrence | TKO (punches) | UFC 150 | August 11, 2012 | 2 | 4:49 | Denver, Colorado, United States | |
| Win | 5–1 | Pat Schilling | Decision (unanimous) | The Ultimate Fighter: Live Finale | June 1, 2012 | 3 | 5:00 | Las Vegas, Nevada, United States | |
| Loss | 4–1 | Dustin Poirier | Submission (triangle armbar) | UFC 143 | February 4, 2012 | 1 | 3:23 | Las Vegas, Nevada, United States | Featherweight debut. |
| Win | 4–0 | Eddie Rincon | Decision (unanimous) | UIC 4: War on the Valley Isle | July 1, 2011 | 3 | 5:00 | Honolulu, Hawaii, United States | |
| Win | 3–0 | Harris Sarmiento | Decision (split) | X-1 World Events 40 | March 12, 2011 | 5 | 5:00 | Honolulu, Hawaii, United States | Won the X-1 Lightweight Championship. |
| Win | 2–0 | Bryson Kamaka | KO (punches) | X-1 World Events 37 | November 6, 2010 | 1 | 3:09 | Honolulu, Hawaii, United States | |
| Win | 1–0 | Duke Saragosa | Decision (unanimous) | X-1 World Events 36 | September 11, 2010 | 3 | 5:00 | Honolulu, Hawaii, United States | Lightweight debut. |
