| # | Portrait | Name | Term start | Term end | Terms | | Party |
| 1 | | William G. George | 1865 | 1868 | 1 | | Republican |
| 2 | | Louis Humphreys (1816–1880) | 1868 | 1872 | 2 | | Republican |
| 3 | | William Miller (1821–1901) | 1873 | 1876 | 2 | | Republican |
| 4 | | Alexander N. Thomas | 1877 | 1878 | 1 | | Republican |
| 5 | | Lucius G. Tong (1842–1908) | 1878 | 1880 | 1 | | Republican |
| 6 | | Levi J. Ham (1805–1887) | 1880 | 1884 | 2 | | Democratic |
| 7 | | George W. Loughman (1846–1909) | 1884 | 1888 | 2 | | Republican |
| 8 | | William H. Longley | 1888 | 1892 | 2 | | Democratic |
| 9 | | David R. Leeper (1832–1900) | 1892 | 1894 | 1 | | Democratic |
| 10 | | D. B. J. Schafer | 1894 | 1898 | 2 | | Republican |
| 11 | | Schuyler Colfax III (1870–1925) | 1898 | 1902 | 2 | | Republican |
| 12 | | Edward J. Fogarty | 1902 | 1910 | 4 | | Democratic |
| 13 | | Charles L. Goetz | 1910 | 1914 | 2 | | Democratic |
| 14 | | Fred W. Keller (1893–1955) | 1914 | 1918 | 2 | | Independent |
| 15 | | Franklin R. Carson (1861–1929) | January 1918 | 1922 | 2 | | Republican |
| 16 | | Eli F. Seebirt (1879–1955) | 1922 | 1926 | 2 | | Republican |
| 17 | | Chester R. Montgomery | 1926 | 1930 | | | Democratic |
| 18 | | William Riley Hinkle (1874–1964) | 1930 | 1935 | | | Democratic |
| 19 | | George W. Freyermuth (1868–1958) | 1935 | 1938 | | | Republican |
| 20 | | Jesse I. Pavey | 1938 | 1945 | | | Democratic |
| 21 | | F. Kenneth Dempsey (1906–1973) | 1945 | 1947 | | | Democratic |
| 22 | | George A. Schock | 1947 | 1952 | | | Democratic |
| 23 | | John Alden Scott (1916–1986) | 1952 | 1956 | 1 | | Republican |
| 24 | | Edward F. Voorde (1910–1960) | January 1, 1956 | September 2, 1960 | 1¼ | | Democratic |
| 25 | | Frank J. Bruggner (1891–1972) | September 1960 | 1964 | ¾ | | Democratic |
| 26 | | Lloyd M. Allen (1919–1989) | 1964 | 1972 | 2 | | Republican |
| 27 | | Jerry Miller | 1972 | 1975 | 1 | | Democratic |
| 28 | | Peter J. Nemeth (born 1942) | 1976 | 1980 | 1 | | Democratic |
| 29 | | Roger O. Parent (born 1938) | January 1980 | January 3, 1988 | 2 | | Democratic |
| 30 | | Joe E. Kernan (1946–2020) | January 3, 1988 | January 3, 1997 | 2¼ | | Democratic |
| 31 | | Steve Luecke (born 1964) | January 3, 1997 | January 1, 2012 | 3¾ | | Democratic |
| 32 | | Pete Buttigieg (born 1982) | January 1, 2012 | January 1, 2020 | 2 | | Democratic |
| 33 | | James Mueller (born 1982) | January 1, 2020 | Incumbent | | | Democratic |