| Name | Office started | Office ended | Party affiliation |
| First vote | Second vote |
| Nicolás de Ribera (El Viejo) | Juan Tello de Guzmán | January 22, 1535 | December 1535 | — |
| Francisco de Godoy | Juan Mogrovejo de Quiñones | January 1, 1536 | December 1536 |
| Sebastián de Torres | Francisco Dávalos | December 29, 1536 | December 1537 |
| Juan de Barbarán | Hernando de Montenegro | January 1, 1538 | December 1538 |
| Domingo de la Presa | Francisco Herrera | January 1, 1539 | 1539 |
| Martín Pizarro | Juan Fernández | 1540 | 1540 |
| Juan de Barrios (absent) | Alonso Palomino (absent) | 1541 | 1541 |
| Alonso Martín de Don Benito | Ruy-Barba-Cabeza de Baca |
| Francisco de Godoy | Francisco Rodríguez | 1542 | 1542 |
| Juan de Barbarán | Pedro Navarrete | 1543 | 1543 |
| Francisco Ampuero | Alonso Palomino | 1544 | 1544 |
| Antonio de Ribera | Pedro Martín de Sicilia | September 27, 1545 | 1545 |
| Antonio de Ribera | Nicolás de Ribera (El Viejo) | January 1546 | 1546 |
| Martín Pizarro | Juan Fernández | 1547 | 1547 |
| Andrés de Cianca | Gregorio de Silva | 1548 | 1548 |
| Pedro de Portocarrero | Ruy-Barba-Cabeza de Baca | January 1, 1549 | 1549 |
| Francisco de Talavera | Sebastián Gonzáles de Merlo | January 1, 1550 | 1550 |
| Jerónimo de Silva | Alonso Martín de Don Benito | January 1, 1551 | 1551 |
| Antonio de León | Martín Pizarro | January 1, 1552 | 1552 |
| Francisco Velásquez de Talavera | Pedro de Zárate | January 1, 1553 | 1553 |
| Nicolás de Ribera (El Viejo) | Diego Pizarro de Olmos | January 1, 1554 | 1554 |
| Rodrigo Niño | Jerónimo de Silva | January 1, 1555 | 1555 |
| Hernando de Montenegro | Ruy-Barba-Cabeza de Baca | January 1, 1556 | 1556 |
| Muñoz Dávila | Jerónimo de Silva | January 1, 1557 | 1557 |
| Vasco de Guevara | Rodrigo Niño | January 2, 1558 | 1558 |
| Jerónimo de Silva | Diego Pizarro de Olmos | January 1, 1559 | 1559 |
| Sebastián Sánchez de Merlo | Gómez Carabantes de Mazuelas | January 1, 1560 | 1560 |
| Lorenzo Estupiñán de Figueroa | Álvaro de Torres | January 1, 1561 | 1561 |
| Francisco Velásquez de Talavera | García de León | January 1, 1562 | 1562 |
| Muñoz Dávila | Antonio de Ribera | January 1, 1563 | 1563 |
| Gómez Carabantes de Mazuelas | Lorenzo Estupiñán de Figueroa | 1564 | 1564 |
| Ordoño de Valencia | Álvaro de Torres | January 1, 1565 | 1565 |
| Francisco Velásquez de Talavera | Juan de Cadahalso Salazar | January 1, 1566 | 1566 |
| Gómez Carabantes de Mazuelas | Bernardo Ruiz | January 1, 1567 | 1567 |
| Álvaro de Torres | Martín Ruiz de Marchena | January 1, 1568 | 1568 |
| Antonio de Rivera | Francisco de Zárate | 1569 | 1569 |
| Juan de Cadahalso Salazar | Juan de la Rinaga | 1570 | 1570 |
| Francisco de Ampuero | Juan Ruiz | January 1, 1571 | 1571 |
| Álvaro de Torres | Diego Porras Sagredo | January 1, 1572 | 1572 |
| Agustín Ramírez de Molina | Pedro de Vega | January 1, 1573 | 1573 |
| Sancho de Ribera | Francisco de Ampuero | January 1, 1574 | 1574 |
| Antonio Navarro | Diego de Porras Sagredo | January 1, 1575 | 1575 |
| Diego de Carvajal y Vargas | Juan de la Rinaga | January 1, 1576 | 1576 |
| Juan Maldonado de Buendía | Agustín Ramírez de la Molina | January 1, 1577 | 1577 |
| Juan de Caldahaso Salazar | Garci Pérez de Salinas | January 1, 1578 | 1578 |
| Pedro de Zarate | Antonio de Meneses | January 1, 1579 | 1579 |
| Juan de Cadahalso Salazar | Diego Porras Sagredo | January 1, 1580 | 1580 |
| Juan Maldonado de Buendía | Francisco de Aliaga de los Ríos | January 1, 1581 | 1581 |