| Character | Actor | Description |
| Alan (1998–present) | Alan Muraoka | A "warm and welcoming character", who's been the proprietor of Hooper's Store since its more contemporary redesign in 1998. |
| Angela (1993–1995) | Angel Jemmott | Part of the "Around-the-Corner" expansion of the 1990s, Angela was a day care worker. She was the wife of Jamal and mother of baby Kayla. |
| Antonio (1971–1972) | Panchito Gómez | The son of Rafael. |
| Armando "Mando" (2013–2015) | Ismael Cruz Córdova | An energetic Puerto Rican Latino writer and "techie [who] loves his gadgets". |
| Billy Tuesday (2004) | Taylor Matalon | Jane Tuesday's "wily and mischievous brother", who seems to always be at the scene of the crime. |
| Bob Johnson (1969–2016) | Bob McGrath | An original cast member and Sesame Street's resident music teacher. |
| Buddy and Jim (1969–1970) | Brandon Maggart and Jim Catusi | "Two bumblers" who appeared in the first season of Sesame Street. |
| Buffy (1975–1981) | Buffy Sainte-Marie | American folk singer who appeared in an "understated" scene about breastfeeding with Big Bird and her infant son Cody. Buffy helped introduce Native American culture to Sesame Street's audience. |
| Carlo (1993–1998) | Carlo Alban | A teenager who worked at Hooper's Store. |
| Celina(1993–1998) | Annette Calud | A regular for five years who was part of the "Around the Corner" expansion. She was a dance teacher. |
| Charlie (2020–present) | Violet Tinnirello | 8-year-old Charlotte, nicknamed "Charlie", who moved to Sesame Street after her parents retired from the military. Her mother is played by Muppet performer Jennifer Barnhart. |
| Chris Robinson (2007–present) | Chris Knowings | A student who works part-time at Hooper's Store. He is the nephew of Gordon and Susan. |
| Christy Robinson (2008, 2011) | Christy Knowings | Chris' twin sister (also in real life); appeared in 3 episodes. |
| Cody (1977) | Dakota Starblanket Wolfchild | Infant son of Buffy Saint-Marie and Sheldon Wolfchild; was breast-fed by his mother on-air, and helped demonstrate sibling rivalry with Big Bird. |
| David (1971–1989) | Northern Calloway | A hip and upbeat individual fond of eccentric hats who worked at Hooper's Store. He eventually took over management of the store after Mr. Hooper's death. |
| Duane (1991–1993) | Donald Faison | One of Jelani's group of friends, along with Tyrone and Lamar, who formed their own after-school company, "Jelani and Company". |
| Edith Ann | Lily Tomlin | A comic, childlike character who appeared in five segments. |
| Ernestine | Lily Tomlin | A nasal-voiced telephone operator. |
| Gabriela "Gabi" Rodriguez (1989–2013) | Bryan Maitland (1989)Brittany and Sarah Beaudry (1989)Morgan DeSena (1989–93)Gabriela Rose Reagan (1989–93)Desiree Casado (1993–2013) | Daughter of Luis and Maria Rodriguez (born 1989), her character was created as part of the show's curriculum about love, marriage, and childbirth. |
| Dr. Gina Jefferson (1986–2015) | Alison Bartlett-O'Reilly | Started on the show as a teenager who worked in Hooper's Store. She ran a daycare center, later became a veterinarian, and adopted a baby named Marco from Guatemala. |
| Gordon Robinson(1969–2016, 2023–present) | Garrett Saunders (1969)Matt Robinson (1969–1972)Hal Miller (1972–1974)Roscoe Orman (1974–2016, 2023–present) | Named for photographer-filmmaker Gordon Parks. He is a science teacher who owned the brownstone "123 Sesame Street" building with his wife Susan and Miles' father. He was the first character introduced in the show's premiere. Davis described him as "a dutiful husband and steady provider, a well-liked and respected figure in the neighborhood." He is also the father figure of Big Bird and all the Sesame Street characters. |
| Grace (1976–1989) | Clarice Taylor | David's grandmother. |
| Hiroshi (1988–1991) | Gedde Watanabe | A Japanese artist. |
| Mr. Harold Hooper (1969–1983) | Will Lee | The original proprietor of Hooper's Store. Lee described Mr. Hooper as "the gruff grocer with the warm heart". Sesame Street dealt with Lee's 1982 death in what Davis called "a landmark broadcast" that aired on Thanksgiving Day, 1983. |
| Jamal (1993–1995) | Jou Jou | A park ranger who was married to Angela and had a baby named Kayla. |
| Jane Tuesday (2004) | Kyla Taub | A 7-year-old private investigator in a series of short films. |
| Jason (1975) | Jason Kingsley | Child with Down syndrome who made 55 appearances on the show from the early-to-mid 1970s. Jason was the son of writer Emily Kingsley, who pushed for more inclusion of people with disabilities on the show. |
| Jelani (1987–1991) | Eugene Byrd | Byrd was hired by producer Dulcy Singer as a part of a curriculum push about race relations. |
| Jennie (1969) | Jada Rowland | Appeared in first episode of Sesame Street. |
| Joey | Joey Calvan | |
| John-John | John Williams III | Best known for his count-to-20 segment with Herry Monster, Gikow said what made him stand out was his "effortless connection to the Muppets and the bold confidence of his delivery." |
| Kayla (1993–1995) | Rachael McDaniel and Syvae McDaniel | Baby daughter of Angela and Jamal. |
| Larry and Phyllis | Alan Arkin and Barbara Dana | Played by the real-life husband and wife, they taught cooperation during season two. |
| Leela (2008–2015) | Nitya Vidyasagar | Indian American character who ran Sesame Street's laundromat. |
| Lillian (1989–1993) | Lillias White | Ran a daycare on Sesame Street. She was portrayed by Broadway performer White. |
| Linda (1971–2002) | Linda Bove | The neighborhood librarian and Bob's girlfriend. Bove said that writer Emily Kingsley "wrote Linda as a person first, then worried about the other stuff", referring to Linda's deafness and use of American Sign Language. |
| Luis Rodriguez (1971–2016) | Emilio Delgado | Davis calls Luis "a tenderhearted Hispanic man"; Luis married Maria in 1988, and his daughter Gabi was born in 1989. For more than four decades, he ran The Fix-It Shop. |
| Marco Jefferson (2006–2014) | Matthew | Gina's adopted son from Guatemala. |
| Maria Rodriguez (1971–2015) | Sonia Manzano | Starting as a teenager and one of the first Latinas on television, Maria is loosely based upon her portrayer's life. Maria married Luis, became co-owner of the Fix-It Shop after working under him, and had a daughter named Gabi. She had a close relationship with Oscar the Grouch, who nicknamed her Skinny. |
| Miguel (1970–1972) | Jaime Sánchez | First Latino cast member. |
| Mike (1989–1991) | Ward Saxton | One of Gordon's students, always after food. A romantic relationship between him and Gina was implied. |
| Mia (2021–present) | Olivia Perez | Nina's niece and the daughter of Dave and Frank, the first married gay couple on Sesame Street. |
| Miles Robinson (1985–2008) | Miles Orman (1985–1995) Kevin Clash (1987) Imani Patterson (1995–2002), Olamide Faison (2003–2008) | The adopted son of Gordon and Susan Robinson (adopted 1985). |
| Molly (1971–1975) | Charlotte Rae | A female mail carrier. She and Bob were very close. |
| Mr. Handford (1989–1998) | Leonard Jackson (1989–1990) David Smyrl (1990–1998) | A retired firefighter who took over ownership of Hooper's Store from David. Jackson played "the grumpier version" of the character, while Smyrl played the "smiling, singing" Mr. Handford. |
| Mr. MacIntosh (1971) | Chester O'Brien | A fruit vendor played by Sesame Street's floor manager. |
| Mr. Noodle | Bill Irwin (1998–2009, 2017–present) | A mime featured in Elmo's World, whose mistakes empower viewers to, as writer Louise Gikow puts it, "call out instructions that allow them to feel smarter than the adult." |
| Mr. Noodle's Brother, Mr. Noodle (2000–2003) | Michael Jeter | Mr. Noodle's brother who appeared in the Elmo's World segments, often with his brother. |
| Mr. Noodle's Sister, Ms. Noodle | Kristin Chenoweth | Mr. Noodle's sister who appeared in Elmo's World. |