| No. | Governor | Term in office | Party | Election | Lt. Governor |
| 1 | | | James Sevier Conway (1796–1855) | September 13, 1836 – November 4, 1840 (did not run) | Democratic | 1836 | Office did not exist |
| 2 | | Archibald Yell (1797–1847) | November 4, 1840 – April 29, 1844 (resigned) | Democratic | 1840 |
| — | | Samuel Adams (1805–1850) | April 29, 1844 – November 9, 1844 (successor took office) | Democratic | President of the Senate acting |
| 3 | | Thomas Stevenson Drew (1802–1879) | November 9, 1844 – January 10, 1849 (resigned) | Democratic | 1844 |
| 1848 |
| — | | Richard C. Byrd (1805–1854) | January 10, 1849 – April 19, 1849 (successor took office) | Democratic | President of the Senate acting |
| 4 | | John Selden Roane (1817–1867) | April 19, 1849 – November 15, 1852 (did not run) | Democratic | 1849 (special) |
| 5 | | Elias Nelson Conway (1812–1892) | November 15, 1852 – November 15, 1860 (term-limited) | Democratic | 1852 |
| 1856 |
| 6 | | | Henry Massey Rector (1816–1899) | November 15, 1860 – November 3, 1862 (resigned) | Independent Democratic | 1860 |
| — | | | Thomas Fletcher (1817–1880) | November 3, 1862 – November 15, 1862 (successor took office) | Democratic | President of the Senate acting |
| 7 | | | Harris Flanagin (1817–1874) | November 15, 1862 – June 1, 1865 (government in exile disestablished) | Independent | 1862 |
| 8 | | Isaac Murphy (d. 1882) | January 20, 1864 – July 2, 1868 (did not run) | Independent | Provisional governor appointed by constitutional convention |
| 1864 | | Calvin C. Bliss |
| 9 | | | Powell Clayton (1833–1914) | July 2, 1868 – March 17, 1871 (resigned) | Republican | 1868 | James M. Johnson (resigned March 14, 1871) |
| Vacant |
| — | | Ozra Amander Hadley (1826–1915) | March 17, 1871 – January 6, 1873 (successor took office) | Republican | President of the Senate acting |
| 10 | | Elisha Baxter (1827–1899) | January 6, 1873 – November 12, 1874 (did not run) | Republican | 1872 | | Volney V. Smith |
| 11 | | | Augustus Hill Garland (1832–1899) | November 12, 1874 – January 11, 1877 (did not run) | Democratic | 1874 | Office did not exist |
| 12 | | William Read Miller (1823–1887) | January 11, 1877 – January 13, 1881 (lost nomination) | Democratic | 1876 |
| 1878 |
| 13 | | Thomas James Churchill (1824–1905) | January 13, 1881 – January 13, 1883 (did not run) | Democratic | 1880 |
| 14 | | James Henderson Berry (1841–1913) | January 13, 1883 – January 15, 1885 (did not run) | Democratic | 1882 |
| 15 | | Simon Pollard Hughes Jr. (1830–1906) | January 15, 1885 – January 17, 1889 (lost nomination) | Democratic | 1884 |
| 1886 |
| 16 | | James Philip Eagle (1837–1904) | January 17, 1889 – January 14, 1893 (did not run) | Democratic | 1888 |
| 1890 |
| 17 | | William Meade Fishback (1831–1903) | January 14, 1893 – January 18, 1895 (did not run) | Democratic | 1892 |
| 18 | | James Paul Clarke (1854–1916) | January 18, 1895 – January 18, 1897 (did not run) | Democratic | 1894 |
| 19 | | Daniel Webster Jones (1839–1918) | January 18, 1897 – January 18, 1901 (did not run) | Democratic | 1896 |
| 1898 |
| 20 | | Jeff Davis (1862–1913) | January 18, 1901 – January 18, 1907 (did not run) | Democratic | 1900 |
| 1902 |
| 1904 |
| 21 | | John Sebastian Little (1851–1916) | January 18, 1907 – February 11, 1907 (resigned) | Democratic | 1906 |
| — | | John Isaac Moore (1856–1937) | February 11, 1907 – May 14, 1907 (legislature adjourned) | Democratic | President of the Senate acting |
| — | | Xenophon Overton Pindall (1873–1935) | May 14, 1907 – January 11, 1909 (senate term expired) | Democratic | President of the Senate acting |
| — | | Jesse M. Martin (1877–1915) | January 11, 1909 – January 14, 1909 (successor took office) | Democratic | President of the Senate acting |
| 22 | | George Washington Donaghey (1856–1937) | January 14, 1909 – January 16, 1913 (lost nomination) | Democratic | 1908 |
| 1910 |
| 23 | | Joseph Taylor Robinson (1872–1937) | January 16, 1913 – March 8, 1913 (resigned) | Democratic | 1912 |
| — | | William Kavanaugh Oldham (1865–1938) | March 8, 1913 – March 13, 1913 (new president of the senate elected) | Democratic | President of the Senate acting |
| — | | Junius Marion Futrell (1870–1955) | March 13, 1913 – August 6, 1913 (successor took office) | Democratic | President of the Senate acting |
| 24 | | George Washington Hays (1863–1927) | August 6, 1913 – January 10, 1917 (did not run) | Democratic | 1913 (special) |
| 1914 |
| 25 | | Charles Hillman Brough (1876–1935) | January 10, 1917 – January 12, 1921 (did not run) | Democratic | 1916 |
| 1918 |
| 26 | | Thomas Chipman McRae (1851–1929) | January 12, 1921 – January 14, 1925 (did not run) | Democratic | 1920 |
| 1922 |