| Route | Start | End | Withdrawal date(s) | Notes |
| 9H | Kensington High Street | Trafalgar Square | 25 July 2014 | Heritage route that used AEC Routemaster buses. |
| 10 | Hammersmith bus station | London King's Cross railway station | 24 November 2018 | Partially replaced by route 23. |
| 15H | Tower Hill tube station | Trafalgar Square | 14 November 2020 | Heritage route that used AEC Routemaster buses. |
| 48 | London Bridge bus station | Walthamstow bus station | 12 October 2019 | Replaced by routes 26, 55 and 388. |
| 77A | Aldwych | Wandsworth | 3 June 2006 | Renumbered 87. |
| 82 | North Finchley bus station | Victoria bus station | 1 April 2017 | Replaced by route 13. |
| 87 | Barking | Romford Market | 25 March 2006 | Replaced by an extension of route 5. |
| 118 | Morden tube station | Brixton tube station | 1 February 2025 | Replaced by route 45. |
| 129 | Claybury Broadway | Becontree Heath | 26 June 2004 | Replaced by revised versions of routes 128 and 150. |
| 143D | Christ's College Finchley | Archway tube station | 4 February 2023 | |
| 168 | Burgess Park | Hampstead Heath | 30 September 2023 | Replaced by route 1. |
| 239 | London Victoria station | Clapham Junction | 16 February 2008 | Replaced by route 170. |
| 271 | Finsbury Square | Highgate Village | 4 February 2023 | Replaced by routes 21, 263 and N271. |
| 305 | Edgware bus station | Kingsbury | 1 September 2018 | Replaced by route 303. |
| 332 | Brent Park | London Paddington station | 29 April 2023 | Replaced by route 16. |
| 347 | Romford railway station | Ockendon railway station | 18 January 2025 | Partially replaced by an extension of route 346. |
| 351 | Bromley North railway station | Penge | 24 June 2002 | Renumbered 354. |
| 369 | Ilford | Thames View Estate | 20 February 2010 | Replaced by new routes EL1 and EL2. |
| 374 | Romford railway station | Harold Hill | 14 October 2005 | Replaced by route 174. |
| 387 | Little Heath | Barking Riverside | 18 February 2017 | Renumbered EL3. |
| 391 | Richmond | Hammersmith bus station | 12 December 2020 | Replaced by route 110. |
| 405D | Purley | West Croydon bus station | 4 January 2022 | Replaced by route 645. |
| 414 | Putney Bridge tube station | Marble Arch tube station | 22 February 2025 | Replaced by a frequency increase on route 14. |
| 455 | Purley | Wallington railway station | 2 March 2024 | Replaced by routes 166, 312 and S4. |
| 497 | Harold Wood railway station | Harold Hill | 9 March 2024 | Replaced by an extension of route 346. |
| 507 | London Waterloo station | Victoria bus station | 29 April 2023 | Replaced by routes 3, 11 and C10. |
| 521 | London Bridge bus station | London Waterloo station | 29 April 2023 | Replaced by routes 59 and 133. |
| 530 | Holloway | Angel tube station | 29 October 2017 | A temporary route introduced on 16 August 2017 and operated by Go-Ahead London. Created due to works on Upper Street.Ran one way to Angel tube station only. |
| 541 | Prince Regent DLR station | Keir Hardie Estate | 1 June 201231 March 201330 October 201519 February 2016 | A temporary route introduced on 3 May 2011, 1 October 2012, 14 September 2013 and 15 February 2016 and operated by Stagecoach London (3 May 2011 until 1 June 2012 and 1 October 2012 until 31 March 2013) and Go-Ahead London (14 September 2013 until 30 October 2015 and 15 February 2015 until 19 February 2016). Created multiple times due to a temporary withdrawal of route 241 between Canning Town bus station and Prince Regent DLR station. |
| 546 | Keston | Downe | 20 December 2024 | A temporary route introduced on 18 November 2024 and operated by Ensignbus. Created due to roadworks on Rookery Road and a temporary withdrawal of route 146 between Keston and Downe. |
| 549 | Loughton tube station | South Woodford tube station | 7 September 2024 | Replaced by a restructured version of route W14. |
| 558 | Seven Sisters station | Chingford Mount | 28 August 2015 | A temporary route introduced on 10 August 2015 and operated by Metroline. Created due to a closure of the Victoria line between Seven Sisters and Walthamstow Central stations. |
| 588 | Hackney Wick | Stratford City bus station | 14 December 2013 | A temporary route introduced on 13 July 2013 and operated by Stagecoach London. Was the first bus route to operate through the Queen Elizabeth Olympic Park. |
| 605 | Burnt Oak tube station | Totteridge & Whetstone tube station | 24 July 2025 | |
| 607 | Uxbridge tube station | White City bus station | 15 July 2023 | Renumbered SL8. |
| 609 | Hammersmith bus station | Mortlake | 18 May 2019 | Afternoon journeys to Hammersmith bus station started from The Harrodian School. |
| 611 | Stonebridge Park station | Christ's College Finchley | 18 July 2020 | Partially replaced by route 112. |
| 618 | Mill Hill Broadway railway station | Avanti House Secondary School | 3 April 2018 | |
| 619 | Edgware bus station | Avanti House Secondary School | 3 April 2018 | |
| 636 | Kemnal Technology College | Grove Park | 2 September 2017 | Ran one way to Grove Park only. |
| 637 | Kemnal Technology College | Grove Park | 2 September 2017 | Ran one way to Grove Park only. |
| 641 | West Molesey | Teddington School | 30 September 2017 | |
| 647 | Romford railway station | Drapers' Academy | 5 September 2016 | Replaced by route 496. |
| 648 | Romford railway station | Cranham | 24 July 2021 | Replaced by route 248 and partially replaced by route 646. |
| 654 | Addington Village Interchange | Ramsden | 24 July 2025 | |
| 673 | The Warren School | Beckton bus station | 24 July 2025 | Ran one way to Beckton bus station only. |
| 689 | Norwood bus garage | Burntwood School | 30 July 2011 | Replaced by route 690. |
| 692 | Dame Alice Owen's School | Southgate tube station | 2 September 2023 | Ran one way to Southgate tube station only. |
| 700 | Colliers Wood tube station | Mitcham | 14 September 2025 | A temporary route introduced on 14 July 2025 and operated by Go-Ahead London. Created due to road closures in Mitcham. |