| Order | Launch date | Mission | Shuttle | Crew | Duration | Launch pad | Landing site | Notes | Refs. |
| 1 | 12 April 198112:00:04 UTC07:00:04 EST | STS-1 | Columbia | 2 | 02d 06h | LC-39A | Edwards | First orbital flight test First reusable orbital spacecraft flight Maiden flight of Columbia Maiden flight of the Space Shuttle program | |
| 2 | 12 November 198115:10:00 UTC10:10:00 EST | STS-2 | Columbia | 2 | 02d 06h | LC-39A | Edwards | Second orbital flight test First reuse of a crewed orbital space vehicle First test of Canadarm robot arm Truncated due to fuel cell problem Shortest orbital mission flown | |
| 3 | 22 March 198216:00:00 UTC11:00:00 EST | STS-3 | Columbia | 2 | 08d 00h | LC-39A | White Sands | Third orbital test Only landing at White Sands, New Mexico | |
| 4 | 27 June 198215:00:00 UTC11:00:00 EDT | STS-4 | Columbia | 2 | 07d 01h | LC-39A | Edwards | Fourth and final orbital flight test First Department of Defense (DoD) payload | |
| 5 | 11 November 198212:19:00 UTC07:19:00 EST | STS-5 | Columbia | 4 | 05d 02h | LC-39A | Edwards | First "operational" flight Multiple comsat deployments First Extravehicular activity of program canceled due to suit problems | |
| 6 | 4 April 198318:30:00 UTC13:30:00 EST | STS-6 | Challenger | 4 | 05d 00h | LC-39A | Edwards | Tracking and data relay satellite (TDRS-A) deployment Maiden flight of Challenger First Space Shuttle Extravehicular activity | |
| 7 | 18 June 198311:33:00 UTC07:33:00 EDT | STS-7 | Challenger | 5 | 06d 02h | LC-39A | Edwards | First American woman in space, Sally Ride Multiple comsat deployments First deployment and retrieval of a Shuttle pallet satellite | |
| 8 | 30 August 198306:32:00 UTC02:32:00 EDT | STS-8 | Challenger | 5 | 06d 01h | LC-39A | Edwards | Comsat deployment First flight of an African American in space, Guion Bluford Test of robot arm on heavy payloads with Payload Flight Test Article First night launch First night landing | |
| 9 | 28 November 198316:00:00 UTC11:00:00 EST | STS-9 | Columbia | 6 | 10d 07h | LC-39A | Edwards | First Spacelab mission First European Space Agency Astronaut on a shuttle mission, Ulf Merbold | |
| 10 | 3 February 198413:00:00 UTC08:00:00 EST | STS-41-B | Challenger | 5 | 07d 23h | LC-39A | Kennedy SLF | Deployed two Comsats First untethered spacewalk by Bruce McCandless II with Manned Maneuvering Unit First landing at Shuttle Landing Facility at Kennedy Dry run of equipment for Solar Maximum Mission rescue | |
| 11 | 6 April 198413:58:00 UTC08:58:00 EST | STS-41-C | Challenger | 5 | 06d 23h | LC-39A | Edwards | Solar Maximum Mission servicing First satellite rescue by astronauts Long Duration Exposure Facility deployment | |
| 12 | 30 August 198412:41:50 UTC08:41:50 EDT | STS-41-D | Discovery | 6 | 06d 00h | LC-39A | Edwards | Multiple comsat deployments Maiden flight of Discovery Test of OAST-1 Solar Array | |
| 13 | 5 October 198411:03:00 UTC07:03:00 EDT | STS-41-G | Challenger | 7 | 08d 05h | LC-39A | Kennedy SLF | Earth Radiation Budget Satellite deployment First flight of two women in space, Sally Ride and Kathryn Sullivan First spacewalk by American woman, Sullivan First Canadian in space, Marc Garneau | |
| 14 | 8 November 198412:15:00 UTC07:15:00 EST | STS-51-A | Discovery | 5 | 07d 23h | LC-39A | Kennedy SLF | Multiple comsat deployments Retrieval of two other comsats (Palapa B2 and Westar VI), which were subsequently refurbished on Earth and reflown | |
| 15 | 24 January 198519:50:00 UTC14:50:00 EST | STS-51-C | Discovery | 5 | 03d 01h | LC-39A | Kennedy SLF | First classified Department of Defense (DoD) mission Magnum satellite deployment | |
| 16 | 12 April 198513:59:05 UTC08:59:05 EST | STS-51-D | Discovery | 7 | 06d 23h | LC-39A | Kennedy SLF | Multiple comsat deployments First flight of a sitting politician in space, Jake Garn First impromptu Extravehicular activity of program to fix Syncom F3 (Leasat 3) | |
| 17 | 29 April 198516:02:18 UTC12:02:18 EDT | STS-51-B | Challenger | 7 | 07d 00h | LC-39A | Edwards | First mission with Spacelab module in a fully operational configuration Conducted experiments in microgravity First African-American Space Shuttle pilot, Frederick D. Gregory | |
| 18 | 17 June 198511:33:00 UTC07:33:00 EDT | STS-51-G | Discovery | 7 | 07d 01h | LC-39A | Edwards | Multiple comsat deployments Flight of first member of royalty, Saudi, Muslim, and Arab in space, Sultan bin Salman Al Saud | |
| 19 | 29 July 198522:00:00 UTC18:00:00 EDT | STS-51-F | Challenger | 7 | 07d 22h | LC-39A | Edwards | Spacelab mission Abort to Orbit. Faulty temperature sensor incorrectly indicated that fuel turbine discharge temperature exceeded the limit. Therefore, one main engine was shut down at T+345 s, resulting in a much lower orbit than planned. All mission objectives achieved | |
| 20 | 27 August 198510:58:01 UTC06:58:01 EDT | STS-51-I | Discovery | 5 | 07d 02h | LC-39A | Edwards | Multiple comsat deployments Rescue of Syncom F3 (Leasat-3) | |
| 21 | 3 October 198515:15:30 UTC11:15:30 EDT | STS-51-J | Atlantis | 5 | 04d 01h | LC-39A | Edwards | Second classified DoD mission Defense Satellite Communications System satellite deployment Maiden flight of Atlantis | |
| 22 | 30 October 198517:00:00 UTC12:00:00 EST | STS-61-A | Challenger | 8 | 07d 00h | LC-39A | Edwards | Largest crew on a spaceflight Third flight of Spacelab Spacelab-D1 microgravity experiments Mission funded by West Germany Last successful mission of Challenger First Dutchman in space, Wubbo Ockels | |
| 23 | 26 November 198524:29:00 UTC19:29:00 EST | STS-61-B | Atlantis | 7 | 06d 21h | LC-39A | Edwards | Multiple comsat deployment EASE/ACCESS experiment First Mexican in space, Rodolfo Neri Vela | |
| 24 | 12 January 198611:55:00 UTC06:55:00 EST | STS-61-C | Columbia | 7 | 06d 02h | LC-39A | Edwards | Comsat deployment Flight of US Representative Bill Nelson Second African-American Space Shuttle pilot, Charles Bolden Last successful mission before STS-51-L | |
| 25 | 28 January 198616:38:00 UTC11:38:00 EST | STS-51-L | Challenger | 7 | 00d 00h 01m 13s | LC-39B | Did not land | Planned tracking and data relay satellite (TDRS-B) deployment Teacher in space flight First Space Shuttle launch from LC-39B SRB leak destroyed the orbiter and killed crew | |
| 26 | 29 September 198815:37:00 UTC11:37:00 EDT | STS-26 | Discovery | 5 | 04d 01h | LC-39B | Edwards | Tracking and data relay satellite (TDRS-C) deployment First post-Challenger flight First Space Shuttle mission with an all-veteran crew | |
| 27 | 2 December 198814:30:34 UTC09:30:34 EST | STS-27 | Atlantis | 5 | 04d 09h | LC-39B | Edwards | Third classified DoD mission Lacrosse 1 deployment Heavy damage to the thermal protection system resulted in extreme heat damage to the right wing | |
| 28 | 13 March 198914:57:00 UTC09:57:00 EST | STS-29 | Discovery | 5 | 04d 23h | LC-39B | Edwards | Tracking and data relay satellite (TDRS-D) deployment IMAX camera Space Station Heat Pipe Advanced Radiator Element I space station radiator experiment | |
| 29 | 4 May 198918:46:59 UTC14:46:59 EDT | STS-30 | Atlantis | 5 | 04d 00h | LC-39B | Edwards | Magellan Venus probe deployment First post-Challenger flight to carry a female astronaut | |
| 30 | 8 August 198912:37:00 UTC08:37:00 EDT | STS-28 | Columbia | 5 | 05d 01h | LC-39B | Edwards | Fourth classified DoD mission Satellite Data System deployment | |
| 31 | 18 October 198916:53:40 UTC12:53:40 EDT | STS-34 | Atlantis | 5 | 04d 23h | LC-39B | Edwards | Galileo Jupiter probe deployment IMAX camera | |
| 32 | 22 November 198924:23:30 UTC19:23:30 EST | STS-33 | Discovery | 5 | 05d 00h | LC-39B | Edwards | Fifth classified DoD mission Deployment of Magnum First African-American Space Shuttle commander, Frederick D. Gregory | |
| 33 | 9 January 199012:35:00 UTC07:35:00 EST | STS-32 | Columbia | 5 | 10d 21h | LC-39A | Edwards | Syncom IV-F5 satellite deployment Long Duration Exposure Facility retrieval IMAX camera | |
| 34 | 28 February 199007:50:22 UTC02:50:22 EST | STS-36 | Atlantis | 5 | 04d 10h | LC-39A | Edwards | Sixth classified DoD mission Misty reconnaissance satellite deployment | |
| 35 | 24 April 199012:33:51 UTC08:33:51 EDT | STS-31 | Discovery | 5 | 05d 01h | LC-39B | Edwards | Hubble Space Telescope (HST) deployment | |
| 36 | 6 October 199011:47:15 UTC07:47:15 EDT | STS-41 | Discovery | 5 | 04d 02h | LC-39B | Edwards | Ulysses/Inertial Upper Stage solar probe deployment | |
| 37 | 15 November 199023:48:15 UTC18:48:15 EST | STS-38 | Atlantis | 5 | 04d 21h | LC-39A | Kennedy SLF | Seventh classified DoD mission Likely SDS2-2 deployed | |
| 38 | 2 December 199006:49:01 UTC01:49:01 EST | STS-35 | Columbia | 7 | 08d 23h | LC-39B | Edwards | Use of ASTRO-1 observatory | |
| 39 | 5 April 199114:22:45 UTC09:22:45 EST | STS-37 | Atlantis | 5 | 05d 23h | LC-39B | Edwards | Compton Gamma Ray Observatory deployment and EVA repair | |
| 40 | 28 April 199111:33:14 UTC07:33:14 EDT | STS-39 | Discovery | 7 | 08d 07h | LC-39A | Kennedy SLF | First unclassified DoD mission (eighth DoD mission overall) Military science experiments | |
| 41 | 5 June 199113:24:51 UTC09:24:51 EDT | STS-40 | Columbia | 7 | 09d 02h | LC-39B | Edwards | Spacelab mission First space mission with three women | |
| 42 | 2 August 199115:02:00 UTC11:02:00 EDT | STS-43 | Atlantis | 5 | 08d 21h | LC-39A | Kennedy SLF | Tracking and data relay satellite (TDRS-E) deployment | |
| 43 | 12 September 199123:11:04 UTC19:11:04 EDT | STS-48 | Discovery | 5 | 05d 08h | LC-39A | Edwards | Upper Atmosphere Research Satellite deployment | |
| 44 | 24 November 199123:44:00 UTC18:44:00 EST | STS-44 | Atlantis | 6 | 06d 22h | LC-39A | Edwards | Second unclassified DoD mission (ninth DoD mission overall) DSP satellite deployment | |
| 45 | 22 January 199214:52:33 UTC09:52:33 EST | STS-42 | Discovery | 7 | 08d 01h | LC-39A | Edwards | Spacelab mission First Canadian woman in space, Roberta Bondar | |
| 46 | 24 March 199213:13:40 UTC08:13:40 EST | STS-45 | Atlantis | 7 | 08d 22h | LC-39A | Kennedy SLF | ATLAS-1 science platform Second African-American Space Shuttle commander, Charles Bolden | |
| 47 | 7 May 199223:40:00 UTC19:40:00 EDT | STS-49 | Endeavour | 7 | 08d 21h | LC-39B | Edwards | Intelsat VI hand-retrieval and repair Maiden flight of Endeavour First three-person Extravehicular activity Assembly of Station by EVA Methods (ASEM) space station truss experiment EVA Record four EVAs total for mission First landing with a drag chute | |
| 48 | 25 June 199216:12:23 UTC12:12:23 EDT | STS-50 | Columbia | 7 | 13d 19h | LC-39A | Kennedy SLF | Spacelab mission | |
| 49 | 31 July 199213:56:48 UTC09:56:48 EDT | STS-46 | Atlantis | 7 | 07d 23h | LC-39B | Kennedy SLF | Deployment of EURECA Deployment of National Aeronautics and Space Administration/Italian Space Agency Tethered Satellite System (TSS) | |