| District | County(ies) represented | Portrait | Senator | Party | Firstelected | Standingcommitteeleader | Appropriationssubcommitteemember |
| 1 | Woodbury | | Catelin Drey | Democratic | 2025 | TBA | TBA |
| 2 | Plymouth and Sioux | | Jeff Taylor | Republican | 2020 | Education (Vice Chair) | Education (Chair) |
| 3 | Osceola, O'Brien, Clay, Cherokee, and Buena Vista | | Lynn Evans | Republican | 2022 | | Education |
| 4 | Calhoun, Pocahontas, Sac, and Webster | | Tim Kraayenbrink | Republican | 2014 | Appropriations (Chair), Technology (Vice Chair) | |
| 5 | Clay, Dickinson, Emmet, Kossuth, Palo Alto and Winnebago | | Dave Rowley | Republican | 2020 | | Administration and Regulation Appropriations (Chair) |
| 6 | Audubon, Carroll, Crawford, Ida, and Shelby | | Jason Schultz | Republican | 2014 | State Government (Chair) | |
| 7 | Cherokee, Monona, Plymouth, and Woodbury | | Kevin Alons | Republican | 2022 | | Health and Human Services |
| 8 | Fremont, Harrison, Mills, and Pottawattamie, | | Mark Costello | Republican | 2014 | Ethics (Vice Chair) | Health and Human Services (Chair) |
| 9 | Adams, Cass, Montgomery, Page, Ringgold, Taylor, and Union | | Tom Shipley | Republican | 2014 | Ethics (Chair), Natural Resources and Environment (Vice Chair) | Agriculture and Natural Resources |
| 10 | Pottawattamie | | Dan Dawson | Republican | 2016 | Ways and Means (Chair) | |
| 11 | Marion and Warren | | Julian Garrett | Republican | 2013 | Judiciary (Vice Chair) | Justice System (Chair) |
| 12 | Adair, Appanoose, Clarke, Dallas, Decatur, Lucas, Madison, Union and Wayne | | Amy Sinclair | Republican | 2012 | Government Oversight (Chair), Rules and Administration (Vice Chair) | |
| 13 | Appanoose, Davis, Monroe, and Wapello | | Cherielynn Westrich | Republican | 2022 | | Justice System (Vice Chair) |
| 14 | Dallas | | Sarah Trone Garriott | Democratic | 2020 | Health and Human Services (Ranking Member) | Health and Human Services |
| 15 | Polk | | Tony Bisignano | Democratic | 2014 | State Government (Ranking Member), Agriculture (Ranking Member) | |
| 16 | Dallas and Polk | | Vacant | | | | |
| 17 | Polk | | Izaah Knox | Democratic | 2022 | Natural Resources and Environment (Ranking Member) | Education |
| 18 | Polk | | Janet Petersen | Democratic | 2012 | Appropriations (Ranking Member) | Transportation, Infrastructure, and Capitals (Ranking Member) |
| 19 | Jasper, Mahaska, and Marion | | Ken Rozenboom | Republican | 2012 | Agriculture (Vice Chair), Education (Chair) | |
| 20 | Polk | | Mike Pike | Republican | 2024 | | |
| 21 | Polk | | Mike Bousselot | Republican | 2022 | Commerce (Vice Chair) | Transportation, Infrastructure, and Capitals (Vice Chair) |
| 22 | Polk | | Matt Blake | Democratic | 2024 | | |
| 23 | Dallas and Polk | | Jack Whitver | Republican | 2011 | Rules and Administration (Chair) | |
| 24 | Boone, Dallas, Greene, Guthrie, and Story | | Jesse Green | Republican | 2020 | Local Government (Chair) | |
| 25 | Story | | Herman Quirmbach | Democratic | 2002 | Education (Ranking Member) | Economic Development |
| 26 | Marshall and Story | | Kara Warme | Republican | 2024 | | |
| 27 | Black Hawk, Grundy, Hardin, Poweshiek, and Tama | | Annette Sweeney | Republican | 2018 | Natural Resources and Environment (Chair) | Agriculture and Natural Resources (Vice Chair) |
| 28 | Franklin, Hancock, Hamilton, Humbolt, and Wright | | Dennis Guth | Republican | 2012 | N/A | Administration and Regulation (Vice Chair) |
| 29 | Bremer, Butler, Chickasaw, and Floyd | | Sandy Salmon | Republican | 2022 | Veterans Affairs (Vice Chair) | Justice System |
| 30 | Cerro Gordo, Floyd, Mitchell, and Worth | | Doug Campbell | Republican | 2024 | | |
| 31 | Black Hawk | | William Dotzler | Democratic | 2002 | Veterans Affairs (Ranking Member) | Economic Development (Ranking Member) |
| 32 | Allamakee, Clayton, Fayette, Howard, and Winneshiek | | Mike Klimesh | Republican | 2020 | Government Oversight (Vice Chair); Transportation (Chair) | Health and Human Services |
| 33 | Dubuque, Jones and Jackson | | Carrie Koelker | Republican | 2018 | Was and Means (Vice Chair) | Transportation, Infrastructure, and Capitals (Chair) |
| 34 | Black Hawk, Buchanan, Delaware, Dubuque, and Fayette | | Dan Zumbach | Republican | 2012 | Appropriations(Vice Chair) | Agriculture and Natural Resources (Chair) |
| 35 | Clinton, Jackson, and Scott | | Mike Zimmer | Democratic | 2025 | | |
| 36 | Dubuque | | Thomas Townsend | Democratic | 2024 | | |
| 37 | Linn | | Molly Donahue | Democratic | 2022 | Workforce (Ranking Member) | Health and Human Services (Ranking Member) |
| 38 | Benton, Black Hawk, and Tama | | Dave Sires | Republican | 2024 | | |
| 39 | Linn | | Liz Bennett | Democratic | 2022 | Technology (Ranking Member) | Transportation, Infrastructure, and Capitals |
| 40 | Linn | | Art Staed | Democratic | 2024 | | |
| 41 | Cedar, Muscatine, and Scott | | Kerry Gruenhagen | Republican | 2022 | | Economic Development |
| 42 | Benton and Linn | | Charlie McClintock | Republican | 2022 | Workforce (Vice Chair) | Justice System |
| 43 | Johnson | | Zach Wahls | Democratic | 2018 | Rules and Administration (Ranking Member) | |
| 44 | Henry, Jefferson, Keokuk, Mahaska, and Van Buren | | Adrian Dickey | Republican | 2021 | Workforce (Chair); Transportation (Vice Chair) | Economic Development |
| 45 | Johnson | | Janice Weiner | Democratic | 2022 | Local Government (Ranking Member) | Agriculture and Natural Resources |
| 46 | Iowa, Johnson and Washington | | Dawn Driscoll | Republican | 2020 | Agriculture (Chair) | |
| 47 | Scott | | Scott Webster | Republican | 2022 | | Administration and Regulation |
| 48 | Des Moines, Henry, Louisa, and Muscatine | | Mark Lofgren | Republican | 2016 | Local Government (Vice Chair) | Economic Development (Chair) |
| 49 | Scott | | Cindy Winckler | Democratic | 2022 | Ethics (Ranking Member) | Education (Ranking Member) |
